What they used it for (initial studies) Two Molecular Cell papers from the Sylvester team applied rPRO-seq to the Integrator complex: In neurons, INTS11 emerged as a regulator of genes implicated in neurodevelopmental disorders.
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In stem-cell models, Integrator/INTS11 acts very early in development (as early as embryonic day 2) and likely at transcription initiation, revising prior views.
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Practical notes & limitations It’s not single-cell; think low-input bulk. Still requires careful nuclei prep and run-on handling; results are complementary to, not a replacement for, steady-state RNA-seq (you’ll often want both).
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For more on RNAseq and scRNA-seq, follow Rhymes with Haystack albertvilella.substack.com
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