Just replaced my lens for that very reason. 😡

At least they are a few grand and not the cost of replacing a dropped 100x TIRF objective

@imcf-lex.bsky.social expect an email from my lawyer* *(I'm my lawyer)

There’s a special hell for people who move the sample TOWARDS the objective lens when focusing. This might be a metallographic nightmare but the crunching noise aa the objective’s front lens is crushed is not fun.

As a biotech guy who does product development, wanna push back a little if ordinary users keep making an $$ mistake, then it is the manufacturer that is at fault, ususally maybe not here

But wouldnt it be great if a Really Loud Alarm came on when the oil bottle was lifted while any non-oil objective is being used? You know, loud enough to cause a near-fatal cardiac incident and significant amount of public shame 😁

You know what’ll get that right off? BABB

Seriously, tho; do NOT do this

www.youtube.com/watch?v=fN8X...

There's a whole channel.

Oh yes

Being friends with the head of our imaging facility, I learned that 9 out of 10 times blurry images at the 20x air objective are not the result of a damaged lens but of a fine crust of immersion oil on or within the objective.

I dislike people that do not care about what happens to the objectives tbh

-50 DKP

Upright or inverted? Because let me tell you, I’ve seen that happen on an inverted scope and it quickly demonstrates the difference between “oil on” and “oil in” the objective.

Inverted :')

You don't understand, NA must be increased at any cost!

[expletive]

I guide each photon to the aperture by hand

I'm about to start in a microscopy core and I'm so excited to see all the places immersion oil can go!

🫠

More than one core has substituted all the 20x to multi-immersion because that’s what they all become anyway.

Filled with hungry rodents.

oh so what's going to replace the oil, solar power? What about at night checkmate lieberals.