Thank you for your comments and thoughts! In the paper we tested whether the MusD will respond to enhancers at 3 other developmental loci and it does! So we don’t think it is related to Fgf8 landscape but rather the MusD promoter itself
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Also, in Bl6 mice, the MusD is methylated and silenced despite being inserted at the same position (figure 1 and reported previously in Kano et al.)- this is caused by a polymorphism between mouse strains (what exactly is still under investigation 😉)
jul 11, 2025, 1:26 pm • 0 0 • view
Quite possible - but the repression could still be Polycomb-mediated, especially since you notice that the MusD demethylation is constitutive there. And many developmental loci have Polycomb marks that spread widely from the target gene itself.
jul 11, 2025, 1:29 pm • 2 0 • view